Progesterone action on the human sperm surface is potentiated by an egg-associated acrosin activator
نویسندگان
چکیده
منابع مشابه
Stimulation of protein tyrosine phosphorylation by a progesterone receptor on the cell surface of human sperm.
Mature human sperm initiate a rapid Ca2+ influx and the acrosomal exocytosis in response to progesterone. Recent evidence indicates that both events can be induced by antibody-mediated cross-linking of a sperm surface progesterone receptor. In many other systems in which signal is generated by receptor cross-linking, protein phosphorylation on tyrosine residues is involved in the signal transdu...
متن کاملPurification of mouse sperm acrosin, its activation from proacrosin and effect on homologous egg investments.
When proacrosin from mouse epididymal spermatozoa was activated a single form of acrosin was produced. The enzyme was isolated by gel filtration followed by affinity chromatography using Sepharose-4B linked to an acrosin inhibitor p-(p'-aminophenoxypropoxy)benzamidine. The molecular weight of partly purified acrosin was 53 000 by gel filtration, and of the pure enzyme 39 000 by SDS-polyacrylami...
متن کاملThe sperm chemoattractant secreted from human cumulus cells is progesterone.
BACKGROUND Human spermatozoa appear to be guided by chemotaxis to the oocyte in the female genital tract. While one of the sources of sperm chemoattractants is the cumulus cells that surround the oocyte, the identity of the chemoattractant secreted from them is unknown. Progesterone, recognized to be secreted from cumulus cells, was demonstrated, at the pM concentration range, to be a chemoattr...
متن کاملThe non-genomic action of progesterone in human sperm - an ionotropic rather than metabotropic mechanism
The catalytic mechanism of several small GTPases by their respective GAPs is studied spatio-temporal resolved in great detail. Time-resolved FTIR difference spectroscopy is used to monitor protein reactions and interactions at atomic detail with ms time-resolution [1]). This provides in combination with structural models and biomolecular simulations (Molecular Dynamic and QM/MM) also spatial re...
متن کاملAcrosin activation follows its surface exposure and precedes membrane fusion in human sperm acrosome reaction.
Evidence has accumulated suggesting multiple roles of acrosin in fertilization, including its participation in early steps of gamete recognition and binding. However, the implication of acrosin in many of these processes is not compatible with its presumptive sequestration within the sperm acrosome until a late phase of the acrosome reaction. In an earlier study (J. Tesarik, J. Drahorad, J. Pek...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1993
ISSN: 0014-5793
DOI: 10.1016/0014-5793(93)81780-4